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RESEARCH TOPICS Research projects of IPCB in italian

Mass Spectrometric Characterization of lipopolysaccharides

Lipooligosaccharides (LOS) are powerful Gram-negative glycolipids that evade the immune system and invade host animal and vegetal cells. The structural elucidation of LOS is pivotal to understanding the mechanism of infection at the molecular level. Their amphiphilic nature has always represented the main obstacle for structural analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). Our current approach has solved this important issue allowing us to obtain reflectron MALDI mass spectra of LOS and enabling in-detail characterization of species (also in complex mixtures) with minimal structural differences.

image:Characterization of lipopolysaccharides

The obtained high-quality MALDI mass spectra reveal LOS species characteristic of molecular ions and definite ion fragments originated in the mass spectrometer source. The in-source decay yields B-type ions corresponding to core oligosaccharide(s), and Y-type ions, related to lipid A unit(s). MALDI tandem time-of-flight (TOF/TOF) MS of lipid A allows elucidation of their corresponding structure directly from purified intact LOS, avoiding further chemical manipulations. These findings constitute a significant advancement for the analysis of such valuable biomolecules by expanding MS capabilities within this field.

People to contact: Domenico Garozzo - Luisa Sturiale

Grants 2010-2015
PRIN 2007
PRIN 2009

List of selected publication:

A.Silipo, G.Vitiello, D.Gully, L.Sturiale, C.Chaintreuil, J.Fardoux, D.Gargani, H.I.Lee, G.Kulkarni, N.Busset, R.Marchetti, A.Palmigiano, H.Moll, R.Engel, R.Lanzetta, L.Paduano, M.Parrilli, W.S.Chang, O.Holst, D.K.Newman, D.Garozzo, G.D'Errico, E.Giraud, A.Molinaro
Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes
Nature Communications 5, 5106 (2014)

C.De Castro, A.Molinaro, F.Piacente, J.R.Gurnon, L.Sturiale, A.Palmigiano, R.Lanzetta, M.Parrilli, D.Garozzo, M.G.Tonetti, J.L.Van Etten
Structure of N-linked oligosaccharides attached to chlorovirus PBCV-1 major capsid protein reveals unusual class of complex N-glycans
Proceedings of the National Academy of Sciences of The United States of America 110, 13956-13960 (2013)

A.Silipo, A.Molinaro, T.Ieranò, A.De Soyza, L.Sturiale, D.Garozzo, C.Aldridge, P.A.Corris, C.M.Anjam Khan, R.Lanzetta, M.Parrilli
The Complete Structure and Pro-inflammatory Activity of the Lipooligosaccharide of the Highly Epidemic and Virulent Gram-Negative Bacterium Burkholderia cenocepacia ET-12 (Strain J2315)
Chemistry-A European Journal 13, 3501-3511 (2007)

T.Ieranò, A.Silipo, L.Sturiale, D.Garozzo, H.Brookes, C.M.Anjam Khan, C.Bryant, F.K.Gould, P.A.Corris, R.Lanzetta, M.Parrilli, A.De Soyza, A.Molinaro
The structure and proinflammatory activity of the lipopolysaccharide from Burkholderia multivorans and the differences between clonal strains colonizing pre and posttransplanted lungs
Glycobiology 18(11), 871-881 (2008)

A.Molinaro, A.Silipo, C.De Castro, L.Sturiale, G.Nigro, D.Garozzo, M.L.Bernardini, R.Lanzetta, M.Parrilli
Full structural characterization of Shigella flexneri M90T serotype 5 wild-type R-LPS and its ΔgalU mutant: glycine residue location in the inner core of the lipopolysaccharide
Glycobiology 18, 260-269 (2008)

I.Paciello, A.Silipo, L.Lembo-Fazio, L.Curcurù, A.Zumsteg, G.Noël, V.Ciancarella, L.Sturiale, A.Molinaro, M.L.Bernardini
Intracellular Shigella remodels its LPS to dampen the innate immune recognition and evade inflammasome activation
Proceedings of the National Academy of Sciences of The United States of America 110(46), E4345-E4354 (2013)

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